Synthesis, optimization, characterization, anti-oxidant and anti-cancero...

The synthesis and characterization of Ipomoea aquatica herbal extract gold nanoparticles (IA-AuNPs) are of great interest due to their antioxidant and anticancer properties. Current study reflects simplest procedure to synthesize IA-AuNPs. Its MTT and cytotoxicity assay was evaluated in HepG2 cells with an IC50 value of 69.62 ± 5 µg/ml after 24 h of incubation. Apoptosis was done using ROS/, MMP and live-dead cell examination using AO/EtBr staining. In spectrophotometric analysis a single peak was observed at 542 nm which represents the SPR absorption band of gold nanoparticles. The IA-AuNPs were spherical with 5–40 nm size, with an average size of 10 nm, based on HR-TEM and HR-SEM. The existence of gold in IA-AuNPs was confirmed by EDX data. FTIR confirmed presence of various bioactive compounds in IA-AuNPs, with peaks at 3344cm−1, 2897cm−1, 2168cm−1, 1765cm−1, 1640cm−1, and 1275cm−1 corresponding to the-OH group and stretches of CH, C = H, C = O, and CO, respectively. At pH9 and 5Msalt concentration, significant stability of IA-AuNPs was observed. The zeta (ζ) potential value of IA-AuNPs at 25 °C and pH9 was -37.7 mV, showing its good quality and greater stability compared to pH6 and pH7 having zeta (ζ) potential value of -13.1 mV and -20.2 mV respectively. IA-AuNPs exhibited dose-dependent cytotoxicity, and ROS/MMP mediated apoptosis assay. DPPH radical scavenging activities was measured at 517 nm in respect to ascorbic acid in which IA-AuNPs exhibited stronger antioxidant properties than plant extract. Overall, our results suggested that IA-AuNPs have anticancer/ antioxidant properties that may be useful for the treatment of hepatic cancer as future drug.

Optical nanoparticles and functional nanomaterials, which have an ability to transport drugs and detect diseases, have recently attracted considerable interest among scientists. Achieving efficient delivery in both healthy and target cells is of utmost importance. Gold nanoparticles (AuNPs) have received considerable interest because of their unique characteristics, such as ease of synthesis, tunable optical and magnetic properties, controlled surface functionalization, and immobilization [1]. Nano-biotechnology has emerged as a significant subfield in the biotechnology. Nanoparticles can be synthesized in several ways, including thermal decomposition; photochemically mediated reduction into micelles; reduction in solutions; and other chemical, biological, electrochemical, and microwave-based techniques [2]. Currently, "green synthesis of nanoparticles" has attracted attention because they are both cost effective, environment friendly and could be used as potential drug